TCMPG

Primer3

Design PCR primers from DNA sequence.
Use primer3-py integrated with Primer3 to provide a simple and reliable way for users to better automate the design of primers.

Enter Query Sequence
Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored). FASTA format ok.

General Primer Picking Conditions

Primer Num Return:

Product Size Min:

Product Size Max:

Primer Min Size:

Primer Opt Size:

Primer Max Size:

Primer Min GC:

Primer Opt GC:

Primer Max GC:

Primer Min TM:

Primer Opt TM:

Primer Max TM:

Dev Tool